Tag: directed evolution
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Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis
Handal-Marquez P, Nguyen H, Pinheiro VB. Frontiers in Molecular Biosciences (2024).
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Rational evolution for altering the ligand preference of estrogen receptor alpha
Eerlings R, Gupta P, Lee XJ, Nguyen T, El Kharraz S, Handle F, Smeets E, Moris L, Devlies W, Vandewinkel…
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Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality
Chaillou S, Stamou PE, Torres LL, Riesco AB, Hazelton W, Pinheiro VB. Nucleic Acids Research (2022).
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Directed evolution of mesophilic HNA polymerases providing insight into DNA polymerase mechanisms
Handal P, Torres L, Bernardes Pinheiro V. bioRxiv (2022).
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A novel framework for engineering protein loops exploring length and compositional variation
Tizei PAG, Harris E, Withanage S, Renders M, Pinheiro VB. Scientific Reports (2021).
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Bacterial Cell Display as a Robust and Versatile Platform for Engineering Low-Affinity Ligands and Enzymes
Csibra E, Renders M, Pinheiro VB. ChemBioChem (2020).
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Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution
Samson C, Legrand P, Tekpinar M, Rozenski J, Abramov M, Holliger P, Pinheiro VB, Herdewijn P, Delarue M. Biomolecules (2020).
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Kinetic analysis of N-alkylaryl carboxamide hexitol nucleotides as substrates for evolved polymerases
Renders M, Dumbre S, Abramov M, Kestemont D, Margamuljana L, Largy E, Cozens C, Vandenameele J, Bernardes Pinheiro V, Toye…
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Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins
Vanmeert M, Razzokov J, Mirza MU, Weeks SD, Schepers G, Bogaerts A, Rozenski J, Froeyen M, Herdewijn P, Pinheiro V,…
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E. coli surface display of streptavidin for directed evolution of an allylic deallylase
Heinisch T, Schwizer F, Garabedian B, Csibra E, Jeschek M, Vallapurackal J, Pinheiro VB, Marliere P, Panke S, Ward TR.…