In BioRxiv

Tizei P., Harris E., Renders M., Withanage, S. and Pinheiro V.B. (BioRxiv). InDel assembly: A novel framework for engineering protein loops through length and compositional variation. doi: 10.1101/127829

Csibra E., Renders, M., Pinheiro, V.B. (BioRxiv). Towards XNA molecular biology: Bacterial cell display as a robust and versatile platform for the engineering of low affinity ligands and enzymes. doi: 10.1101/2020.01.06.896050

Marquez, H., Koch, M., Kestemont, D., Arangundy-Franklin, S., Pinheiro, V.B. (BioRxiv). Antha-guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries. doi: 10.1101/2019.12.17.879486


Pinheiro, V.B. (2020). Biotechnology Tools Derived from the Bacteriophage/Bacteria Arms Race. In Bacteriophages – Perspectives and Future. doi: 10.5772/intechopen.90367


Pinheiro, V.B. (2019). Engineering-driven biological insights into DNA polymerase mechanism. CURRENT OPINION IN BIOTECHNOLOGY, 60, 9-16. doi: 10.1016/j.copbio.2018.11.008

Vanmeert, M., Razzokov, J., Mirza, M.U., Weeks, S.D., Schepers, G., Bogaerts, A., Rozenski, J., Froeyen, M., Herdewijn, P., Pinheiro, V., Lescrinier, E. (2019). Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins. NUCLEIC ACIDS RESEARCH, 1-12. doi: 10.1093/nar/gkz551

Vecchioni, S., Capece, M.C., Toomey, E., Le, N., Ray, A., Greenberg, A., Fujishima, K., Urbina, J., Paulino-Lima, I.G., Pinheiro, V., Shih, J., Wessel, G., Wind, S.J., Rothschild, L. (2019). Construction and characterization of metal ion-containing DNA nanowires for synthetic biology and nanotechnology. SCIENTIFIC REPORTS, 9, Art.No. ARTN 6942. doi: 10.1038/s41598-019-43316-1

Fedorec, A.J H., Ozdemir, T., Doshi, A., Ho, Y-K., Rosa, L., Rutter, J., Velazquez, O., Pinheiro, V.B., Danino, T., Barnes, C.P. (2019). Two New Plasmid Post-segregational Killing Mechanisms for the Implementation of Synthetic Gene Networks in Escherichia coli. ISCIENCE, 14, 323-+. doi: 10.1016/j.isci.2019.03.019

Renders, M., Dumbre, S., Abramov, M., Kestemont, D., Margamuljana, L., Largy, E., Cozens, C., Vandenameele, J., Pinheiro, V.B., Toye, D., Frère, J-M., Herdewijn, P. (2019). Kinetic analysis of N-alkylaryl carboxamide hexitol nucleotides as substrates for evolved polymerases. NUCLEIC ACIDS RESEARCH, 47 (5), 2160-2168. doi: 10.1093/nar/gkz008


Cozens C., Pinheiro VB. (2018). XNA Synthesis and Reverse Transcription by Engineered Thermophilic Polymerases. Curr Protoc Chem Biol, 10 (3).

Jabgunde AM., Jaziri F., Bande O., Froeyen M., Abramov M., Hoai N., Schepers G., Lescrinier E., Pinheiro VB., Pezo V., Marliere P., Herdewijn P. (2018). Methylated Nucleobases: Synthesis and Evaluation for Base Pairing In Vitro and In Vivo. CHEMISTRY-A EUROPEAN JOURNAL, 24 (48), 12695-12707.

Heinisch T., Schwizer F., Garabedian B., Csibra E., Jeschek M., Vallapurackal J., Pinheiro VB., Marliere P., Panke S., Ward TR. (2018). E. coli surface display of streptavidin for directed evolution of an allylic deallylase. CHEMICAL SCIENCE, 9 (24), 5383-5388.

Kestemont D., Renders M., Leonczak P., Abramov M., Schepers G., Pinheiro VB., Rozenski J., Herdewijn P. (2018). XNA ligation using T4 DNA ligase in crowding conditions. CHEMICAL COMMUNICATIONS, 54 (49), 6408-6411.

Torres LL., Pinheiro VB. (2018). Xenobiotic Nucleic Acid (XNA) Synthesis by Phi29 DNA Polymerase. Curr Protoc Chem Biol, 10 (2).

Liu C., Cozens C., Jaziri F., Rozenski J., Marechal A., Dumbre S., Pezo V., Marliere P., Pinheiro VB.*, Groaz E., Herdewijn P.* (2018). Phosphonomethyl Oligonucleotides as Backbone-Modified Artificial Genetic Polymers. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 140 (21), 6690-6699. * corresponding authors.

Cozens C., Pinheiro VB. (2018). Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis. Nucleic Acids Res, 46 (8).


Tizei PA G., Csibra E., Torres L., Pinheiro VB. (2016). Selection platforms for directed evolution in synthetic biology. BIOCHEMICAL SOCIETY TRANSACTIONS, 44, 1165-1175.

Torres L., Kruger A., Csibra E., Gianni E., Pinheiro VB., (2016). Synthetic biology approaches to biological containment: pre-emptively tackling potential risks. SYNTHETIC BIOLOGY, 60 (4), 393-410.


Renders M., Pinheiro VB. (2015). Catalysing Mirror Life. CHEMBIOCHEM, 16 (6), 899-901.

Bande O., Abou El Asrar R., Braddick D., Dumbre S., Pezo V., Schepers G., Pinheiro VB., Lescrinier E., Holliger P., Marlière P., Herdewijn P. (2015). Isoguanine and 5-Methyl-Isocytosine Bases, In Vitro and In Vivo. Chemistry – a European Journal, 21 (13), 5009-22.

Taylor AI., Pinheiro VB., Smola MJ., Morgunov AS., Peak-Chew S., Cozens C., Weeks KM., Herdewijn P., Holliger P. (2015). Catalysts from synthetic genetic polymers. Nature, 518, 427-430.

2014 and earlier

Pinheiro VB.*, Arangundy-Franklin S., Holliger P. (2014). Compartmentalized Self-Tagging for In Vitro-Directed Evolution of XNA Polymerases. Curr Protoc Nucleic Acid Chem, 57, 9.9.1-9.918. *corresponding author

Pinheiro VB., Holliger P. (2014). Towards XNA nanotechnology: new materials from synthetic genetic polymers. TRENDS IN BIOTECHNOLOGY, 32 (6), 321-328.

Wynne SA., Pinheiro VB., Holliger P., Leslie AG W. (2013). Structures of an Apo and a Binary Complex of an Evolved Archeal B Family DNA Polymerase Capable of Synthesising Highly Cy-Dye Labelled DNA. PLOS ONE, 8 (8), Art.No. ARTN e70892.

Pinheiro VB.*, Loakes D., Holliger P. (2013). Synthetic polymers and their potential as genetic materials. BIOESSAYS, 35 (2), 113-122. *corresponding author

Pinheiro VB., Holliger P. (2012). The XNA world: progress towards replication and evolution of synthetic genetic polymers. CURRENT OPINION IN CHEMICAL BIOLOGY, 16 (3-4), 245-252.

Cozens C., Pinheiro VB., Vaisman A., Woodgate R., Holliger P. (2012). A short adaptive path from DNA to RNA polymerases. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109 (21), 8067-8072.

Pinheiro VB., Taylor AI., Cozens C., Abramov M., Renders M., Zhang S., Chaput JC., Wengel J., Peak-Chew S-Y., McLaughlin SH., Herdewijn P., Holliger P. (2012). Synthetic genetic polymers capable of heredity and evolution. Science, 336 (6079), 341-4.

Attwater J., Wochner A., Pinheiro VB., Coulson A., Holliger P. (2010). Ice as a protocellular medium for RNA replication. NATURE COMMUNICATIONS, 1, Art.No. ARTN 76.

Loakes D., Gallego J., Pinheiro VB., Kool ET., Holliger P. (2009). Evolving a Polymerase for Hydrophobic Base Analogues. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 131 (41), 14827-14837.

Pinheiro VB., Ellar DJ. (2007). Expression and insecticidal activity of Yersinia pseudotuberculosis and Photorhabdus luminescens toxin complex proteins. CELLULAR MICROBIOLOGY, 9 (10), 2372-2380.

Pinheiro VB., Ellar DJ. (2006). How to kill a mocking bug?. CELLULAR MICROBIOLOGY, 8 (4), 545-557.